| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.017497 |
| Chromosome: | chromosome 1 |
| Location: | 4347711 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029500 | CDPKK3 | Calcium/calmodulin-dependent protein kinase kinase; (1 of 1) PTHR24347//PTHR24347:SF249 - SERINE/THREONINE-PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTGCAGGAGTAAAGAACGGAGCAGAGGGAAGGAGAAGGAGCGGTGAGG |
| Internal bar code: | CGCTAGACGCTGGACCGGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2145 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCAACCTGCGCTCCAAGA |
| Suggested primer 2: | TCCGACCGAAAAACCCACAT |