| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.017506 |
| Chromosome: | chromosome 14 |
| Location: | 2089126 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g801627 | (1 of 1) PTHR10438//PTHR10438:SF255//PTHR10438:SF256 - THIOREDOXIN // THIOREDOXIN M4, CHLOROPLASTIC // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACCGACGTTTACCAGGCCAGTTGAAAAACGAGCTGGCGCGAATGAACT |
| Internal bar code: | GTAGGAGTTGCTCGTGTGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3595 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTATTGAGCGCTCGGAGG |
| Suggested primer 2: | GACTGCCTTTGGATCCAGCT |