| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.017532 |
| Chromosome: | chromosome 7 |
| Location: | 6419176 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357750 | (1 of 3) PTHR10383 - SERINE INCORPORATOR | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGCTGGCGATACACCGCAATTGGAGTGAGCGCGGAACGTGATACGCA |
| Internal bar code: | CCGTCTGCCCCCGTCCGGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 950 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGTGACGTGGAACCTCA |
| Suggested primer 2: | GCGTGAGATAGTACAGGGCC |