| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.017534 |
| Chromosome: | chromosome 4 |
| Location: | 3290281 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226850 | ASP1 | (1 of 1) 3.4.23.5 - Cathepsin D; Pepsin-type aspartyl protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAGGCCTAGAGCTAGGCATGGCCTCCAGTTCAGTGTCTCACTAACCG |
| Internal bar code: | TTGTTGAACAATCTCGACCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3053 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACAGGCCTTGCTTCGGAA |
| Suggested primer 2: | TAGGTCTTGCTCTTGGCTGC |