| Insertion cassette: | CIB2 | 
| Side of cassette: | 5' | 
| Strand: | - | 
| Strain: | CLIP2.017538 | 
| Chromosome: | chromosome 12 | 
| Location: | 5129041 | 
| Confidence (%): | 80 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre12.g801426 | (1 of 726) IPR011009 - Protein kinase-like domain | intron | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCATTGACAACGCGCCCGAGCGCCGGCGCACCGGCAAGAACAAGGACA | 
| Internal bar code: | GTGGCTGTACCGCTTCTGTCAA | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3492 | 
| LEAP-Seq percent confirming: | 11.1111 | 
| LEAP-Seq n confirming: | 9 | 
| LEAP-Seq n nonconfirming: | 72 | 
| LEAP-Seq n unique pos: | 81 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCAACACGCTTATGGATG | 
| Suggested primer 2: | CCACAACTCTGTTTGGCGTG |