| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.017544 |
| Chromosome: | chromosome 12 |
| Location: | 8141848 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552150 | (1 of 1) PF00069//PF13185 - Protein kinase domain (Pkinase) // GAF domain (GAF_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACCTTGAACAGGACAACTTTGAACGGACTGGGACGGACGACTCCAAC |
| Internal bar code: | AGAGGCAACTGTCCCGGAAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 73 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGGCTAGCGAACCGTAT |
| Suggested primer 2: | ACACGCACATGTAGCAGTGA |