| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.017546 |
| Chromosome: | chromosome 9 |
| Location: | 1194259 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401000 | SNE3 | UDP-N-acetylglucosamine/UDP-glucose 4-epimerase | CDS |
| Cre09.g401022 | SNE3, UXE,UXE1 | UDP-xylose-4-epimerase; (1 of 1) K12448 - UDP-arabinose 4-epimerase (UXE, uxe) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAACTACCTGCAGCAAGCAGGTTGCTAGGACGAGGGCCTCGATCCTG |
| Internal bar code: | GCCATCTAGCCTATGAATATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 599 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGACGTACAGGAACCGTAC |
| Suggested primer 2: | GGCGAACGATTGTGTATGGC |