Insertion junction: CLIP2.017562_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.017562
Chromosome:chromosome 13
Location:2709299
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g581750 (1 of 3) K10752 - histone-binding protein RBBP4 (RBBP4, HAT2, CAF1, MIS16) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGGGTGCAGCTGCAGCGTCGGGCTGCGGGGGAAGGCGCGGTGGGAGGC
Internal bar code:CGGGACACAAGGTAAGTGAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1707
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GACACCATGGTCCAGGACAG
Suggested primer 2:CATCTGGGTCCTCGTCTTCG