Insertion junction: CLIP2.017604_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.017604
Chromosome:chromosome 1
Location:51406
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g000200 (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain; Esterase, SGNH hydrolase-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGAATTTGCTGCCGGGCGCACCAAAGCGCCGCCTCCCCTTTAGCTCGT
Internal bar code:GCGTAGAAGGAAGACTATGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1212
LEAP-Seq percent confirming:95.4545
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTTCCCCGCTGTCTTCTG
Suggested primer 2:CCTGCCAGCTTGTCAAACAC