Insertion junction: CLIP2.017631_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.017631
Chromosome:chromosome 10
Location:1678552
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g429750 FAP417 (1 of 1) IPR000014//IPR000104 - PAS domain // Antifreeze protein, type I; Flagellar Associated Protein 417 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACGATGTGTACGGCAGGAACAGATTGTACGGTCCTGGAGCTATACTGC
Internal bar code:TGTACCCGACTCCCCCCAACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:3266
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGCCGCAATGACAAACAC
Suggested primer 2:ATTCGAATCGTACGGGAGCC