Insertion junction: CLIP2.017642_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.017642
Chromosome:chromosome 3
Location:3452530
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g166900 CYP767A1,CYP15 (1 of 1) 1.14.13.98 - Cholesterol 24-hydroxylase / Cytochrome P450 46A1; Cytochrome P450, CYP4 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATATTATCATACCAGGGCTCTGGAGGTGATCGCGCTGGTCGTGTGCGTTG
Internal bar code:AGCGAACGGGCGTATGAGTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:478
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCTACCCCAACCCCAAC
Suggested primer 2:CGGACTGGCTGTGTCAATCT