| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.017645 |
| Chromosome: | chromosome 11 |
| Location: | 822053 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467639 | (1 of 1) PF04910 - Transcriptional repressor TCF25 (Tcf25) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCCTGCAGGGGACGGGGAAGACAGCGTGTGTTGGACAACCCAAGAA |
| Internal bar code: | TTGCGACCGTCAGCGCGGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2484 |
| LEAP-Seq percent confirming: | 14.4737 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGCTGTGGGCTAAATCT |
| Suggested primer 2: | GATTCTCCTCCACGCCGTAG |