Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.017659 |
Chromosome: | chromosome 17 |
Location: | 817222 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g701751 | (1 of 39) PTHR13078:SF53 - PROTEIN MAOC-1 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGAGCCCTGGCCCGGCGCGCGCCGTCAAAAAATAGTATAATGGGGGTT |
Internal bar code: | CGCGCTGTTGGCCTCTTTCACC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 4793 |
LEAP-Seq percent confirming: | 93.1034 |
LEAP-Seq n confirming: | 27 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTAACATCCCGACGTAGGC |
Suggested primer 2: | AGGAACTGGAGCGATTGGTG |