| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.017693 |
| Chromosome: | chromosome 3 |
| Location: | 3991199 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171550 | SSA13 | cilia-sensing, structure and/or assembly; (1 of 1) PTHR21706//PTHR21706:SF15 - FAMILY NOT NAMED // TRANSMEMBRANE PROTEIN 65 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGAGGGGTAGAAGAAGGGGAGGCTCTTCGCAAACTTCCACTTTGCGT |
| Internal bar code: | CCGTCAAAGAGGAGCGGACAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2295 |
| LEAP-Seq percent confirming: | 80.4348 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCCTTTTGCCAACTCCG |
| Suggested primer 2: | ACAGGTAGCGATGTGGGTTG |