Insertion junction: CLIP2.017707_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.017707
Chromosome:chromosome 6
Location:6918456
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g296850 FAP81,DLEC1 (1 of 8) IPR008962 - PapD-like; Flagellar Associated Protein 81 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCTGCAGACCGAGGCGCAGTACGACGAGGAGCAGGACGACGAGGGCCA
Internal bar code:ATGCTCCAGTCTTGGTCTGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1095
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCAAGTGCTGAACATGAA
Suggested primer 2:AGGTCAAGACGTTCAGCCTG