| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.017725 |
| Chromosome: | chromosome 12 |
| Location: | 2599860 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801383 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAACAGGCGTGATGGGCCCCTGCCCCCAGCCAAAGCTTAGGGCCGGC |
| Internal bar code: | TGATTTATTAGTCAGGAATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3963 |
| LEAP-Seq percent confirming: | 99.2481 |
| LEAP-Seq n confirming: | 132 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 133 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCCCTGAGAAGAAGCAT |
| Suggested primer 2: | TGCAGGTAACGTCAAGGGTC |