Insertion junction: CLIP2.017735_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.017735
Chromosome:chromosome 16
Location:6056239
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g677920 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAACGGAATGGTTGCCTGCTTGCGCAAACGTGGCCCCTTTCGATCGGC
Internal bar code:TGGCTTGCTGAAATAACAGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:3839
LEAP-Seq percent confirming:99.2424
LEAP-Seq n confirming:131
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:132

Suggested primers for confirmation by PCR

Suggested primer 1:TGACAGGGTCCCGTACATCT
Suggested primer 2:AAGCCAGAGGTGTGATGAGC