| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.017752 |
| Chromosome: | chromosome 2 |
| Location: | 1099046 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080600 | BIP2 | (1 of 2) K09490 - heat shock 70kDa protein 5 (HSPA5, BIP); Endoplasmic reticulum associated Hsp70 protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATTGAAGTGACGTTCGAGGTGGACGCCAACGGTATCCTCACCGTCTCG |
| Internal bar code: | CGAAATTGAGGTCATATTATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1602 |
| LEAP-Seq percent confirming: | 94.9153 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTGTCCTCCTCCTCGA |
| Suggested primer 2: | GGGTGGATGGGAGTTTCTGG |