Insertion junction: CLIP2.017755_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.017755
Chromosome:chromosome 2
Location:5515895
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g114700 CYN17,RSP12 (1 of 1) PTHR11071//PTHR11071:SF193 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 6; Radial spoke protein 12 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCTGGGGACTGGCTGGACTTATGCAGCCTGTCGGCGAATGGGCGTGCG
Internal bar code:CCAGACCTCGAGGTTTTTCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2944
LEAP-Seq percent confirming:98.8095
LEAP-Seq n confirming:83
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:84

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTACGTCTCATCCGGCAG
Suggested primer 2:CATGCTGCAATCGCTGAGTC