| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.017758 |
| Chromosome: | chromosome 17 |
| Location: | 1301557 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705350 | CDPKK2 | (1 of 3) K07359 - calcium/calmodulin-dependent protein kinase kinase (CAMKK); Calcium/Calmodulin Dependent Protein Kinase Kinase Homolog 2 isoform beta putative | intron |
| Cre17.g705400 | POA1 | 20S proteasome alpha subunit A; (1 of 1) K02730 - 20S proteasome subunit alpha 1 (PSMA6) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTCTTAACCGCGCTCGTGCTGCCCTCTTTTAGTCTGCTCACCAAAGCG |
| Internal bar code: | TGGCTTGACACGGTCCGGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 945 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGATTGTGTGCGTGCTGG |
| Suggested primer 2: | TGTCGTCCTGCGTGTTGTAA |