Insertion junction: CLIP2.017801_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.017801
Chromosome:chromosome 4
Location:2329577
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g220850 (1 of 1) 3.6.3.34//3.6.3.43//3.6.3.44 - Iron-chelate-transporting ATPase // Peptide-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAATGAATTTCCCCACGCCACCACAACATCCACAACCGACCGAACTAGAT
Internal bar code:TTCCAGGCAGGAAGAGAGTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:177
LEAP-Seq percent confirming:35.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGGAAGGGTAGAGGACGT
Suggested primer 2:GTTCCAGGTGAGAGGTGGTG