| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.017807 |
| Chromosome: | chromosome 13 |
| Location: | 5099991 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606800 | (1 of 2) PTHR30336//PTHR30336:SF4 - INNER MEMBRANE PROTEIN, PROBABLE PERMEASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTATGCCGTCAGCCGGCAGGACGAATTCGGGCAGCAGACCATCAGCGA |
| Internal bar code: | GTACTTCATGGGTCATGATAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1090 |
| LEAP-Seq percent confirming: | 60.8696 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCCAGACATGTAGCACC |
| Suggested primer 2: | CGTACCCTTCCTCCCGTTTC |