Insertion junction: CLIP2.017811_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.017811
Chromosome:chromosome 7
Location:4763867
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g345700 COQ10 Coenzyme Q-binding protein; (1 of 1) K18588 - coenzyme Q-binding protein COQ10 (COQ10) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTTCGATCGCACGCCAGGTCCAAACCCTCACCTTACCACAGCTTCTTC
Internal bar code:ACCTACTGACGTAGATTGGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1112
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:ATACACTCCACCCCTCTCCC
Suggested primer 2:ACAGTCGCGGAGGATTGTTT