Insertion junction: CLIP2.017841_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.017841
Chromosome:chromosome 2
Location:6239137
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g387100 CGL12,PTST1 (1 of 5) PTHR10343 - 5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT; Protein targeting to starch 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGCTGCTGGGCGCGGCGGGCGCAGCGCGGCCAAGTAAGCCAGACAACA
Internal bar code:ACCGCGATCCAGAACGCCATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:4086
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:104
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:104

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGCTTGTGCAAGTGCTTT
Suggested primer 2:GCGGCTTGTAGAGAATTGCG