| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.017841 |
| Chromosome: | chromosome 2 |
| Location: | 6239143 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387100 | CGL12,PTST1 | (1 of 5) PTHR10343 - 5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT; Protein targeting to starch 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAATAGCACCGCAAACACCTCGCGCTCCGCTGCGAGTGAGTTTGCCTCC |
| Internal bar code: | ATTCCGACGCTTTGTTCCAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3067 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGCTTGTAGAGAATTGCG |
| Suggested primer 2: | CGAGCTTGTGCAAGTGCTTT |