| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.017846 |
| Chromosome: | chromosome 10 |
| Location: | 1517733 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428720 | (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCAGGGGCGTCGGCGGGAGCGGCGCCGGTAGCAGCGGTGGCAGCAGTG |
| Internal bar code: | CGTAAATGAGAATGTCTTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2254 |
| LEAP-Seq percent confirming: | 59.5238 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTCGCAGTTACTTGGCAT |
| Suggested primer 2: | TTGCCATGTCCTTGCTGTCA |