| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.017855 |
| Chromosome: | chromosome 2 |
| Location: | 3213612 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096950 | (1 of 1) PF09444 - MRC1-like domain (MRC1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCATCCACCTCGAACTCCGCCTCCCCCTCCTCCTCCTCCTCGTCCT |
| Internal bar code: | TGTCGGAAAGTGGAACGGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1267 |
| LEAP-Seq percent confirming: | 54.902 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCCGGACGATGGCATCTT |
| Suggested primer 2: | GTGTGTGTGTGTGACGTGTG |