| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.017869 |
| Chromosome: | chromosome 7 |
| Location: | 6108186 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355600 | NTR3 | NADPH dependent thioredoxin reductase 3; (1 of 2) PTHR22912//PTHR22912:SF183 - DISULFIDE OXIDOREDUCTASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGTTTAATGCGTTGCTCCCTCCGTCCCCTTCTGTAATCAACAGTCCG |
| Internal bar code: | ATGGGCTTAAAGTCCTGTTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1225 |
| LEAP-Seq percent confirming: | 41.8605 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCCTGCAGCAGTGTGAG |
| Suggested primer 2: | TCATGAGCATTTGCATGGCG |