| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.017884 |
| Chromosome: | chromosome 13 |
| Location: | 1976000 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801525 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAAGTAGCGGGCTCTATCAACCGCGGGAATGCTTTGGCCTTGCAGCCG |
| Internal bar code: | ACATTAGGGAGACTTCAAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2539 |
| LEAP-Seq percent confirming: | 81.5789 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTGAGTTGTTTGGGCTG |
| Suggested primer 2: | CATCATGCGCTCACGGAAAG |