Insertion junction: CLIP2.017924_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.017924
Chromosome:chromosome 3
Location:5350041
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g183200 CPC1 Central pair microtubule complex 1; (1 of 1) PTHR14919:SF0 - SPERM FLAGELLAR PROTEIN 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGAGATGGAGCGCGAGGAGCTGCGTGTTGAGCTGGCGCTGAGCGAGA
Internal bar code:ATCTGCGAAGTATATGCAGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1289
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGCAACAACACTGGTCG
Suggested primer 2:CGAATGCCGGAAGCTTCATG