Insertion junction: CLIP2.017960_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.017960
Chromosome:chromosome 1
Location:3773181
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g024650 CTL1 carbohydrate-binding protein; (1 of 2) PF00059//PF07714//PF14295 - Lectin C-type domain (Lectin_C) // Protein tyrosine kinase (Pkinase_Tyr) // PAN domain (PAN_4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATGGCCTGTGCGTATGCGCGTGCAGAACAATGAGGGCTGCTTAATGAT
Internal bar code:CGATGGTGGTACGTCGACTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:560
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AGTTTGGCGATGACTGCTGA
Suggested primer 2:CCCACCAAGCAAAGAAACGG