| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.017987 |
| Chromosome: | chromosome 10 |
| Location: | 4384253 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450100 | (1 of 1) K04957 - hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (HCN4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCGCTGGTGCCCCTGCCTGCGGCCTTGCGGTGGTTGCCGTCCTTCC |
| Internal bar code: | CGGGTCATTAGGGTTGCCCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 949 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGATGTGATGGTGGTGCAG |
| Suggested primer 2: | CAATAGCCCAATTGCGTCCG |