| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.018013 |
| Chromosome: | chromosome 12 |
| Location: | 4968796 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524800 | (1 of 4) IPR005819 - Histone H5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCGGCCTCACAGGCTGCCAAGCCCAAGGCTGCTCCCAAGGCCAAGGC |
| Internal bar code: | GGGCTAAGGAGTTTCCGAATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2578 |
| LEAP-Seq percent confirming: | 90.566 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAACCCCTGGTGGTAAGGC |
| Suggested primer 2: | ATTATAATGGCCGGTCGCGT |