| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.018019 |
| Chromosome: | chromosome 1 |
| Location: | 6118920 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043500 | (1 of 1) PTHR20882//PTHR20882:SF14 - FAMILY NOT NAMED // CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCGTGAAGATGGTCGATGGCAGGTTGGCCTGCATTGAAAGCAACGTAG |
| Internal bar code: | TTTTGATGTTCTTTATATTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3333 |
| LEAP-Seq percent confirming: | 82.4324 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCAACGATGCCGCATAAC |
| Suggested primer 2: | GAAAGCCTTGGTTTGCTGGG |