Insertion junction: CLIP2.018021_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.018021
Chromosome:chromosome 16
Location:3097377
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g664750 (1 of 1) IPR000225//IPR016024//IPR021133 - Armadillo // Armadillo-type fold // HEAT, type 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGAACCCCTAGCCTATCACGCGCGGCGTGTCGCTGATAGCGCGCTGAC
Internal bar code:TCCGTAGAGTCGTTAGAGTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1385
LEAP-Seq percent confirming:90.4762
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GATATGAGCTGGCGCAGTCC
Suggested primer 2:TAGGCTAGGCATGCGCATAC