| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.018022 |
| Chromosome: | chromosome 13 |
| Location: | 151192 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g562950 | CDC16,DIV38,APC6 | (1 of 1) K03353 - anaphase-promoting complex subunit 6 (APC6, CDC16); Anaphase promoting complex subunit 6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTAAGGAAACAGTCGCGCGGCAAGGAGAACTGCAGCTGCAAAAGCGCG |
| Internal bar code: | TGGGTGCGATTTTGATAACTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1160 |
| LEAP-Seq percent confirming: | 70.8333 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTTACGAGGGGTCGGGGT |
| Suggested primer 2: | AAACAACAAGTGAAGGCGCC |