| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.018034 |
| Chromosome: | chromosome 2 |
| Location: | 154562 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g074300 | (1 of 1) PF11707 - Ribosome 60S biogenesis N-terminal (Npa1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGGCCCCGCTGCGCGAAAACTGAGATGTGAAATAGTATGTACATTTC |
| Internal bar code: | GTGTGGAACTGCTTTAGTTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 993 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCATATGCGTGTCAGTGC |
| Suggested primer 2: | GTCGCGATTGTGTATGTGCC |