| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.018039 |
| Chromosome: | chromosome 16 |
| Location: | 407461 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693600 | ISG-C4,ISG4,FAP137 | Hydroxyproline-rich Flagellar Associated Protein137; (1 of 1) 2.7.11.1//2.7.11.18 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // [Myosin light-chain] kinase / Smooth-muscle-myosin-light-chain kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGTCGAGTAGCCGTGCTTTACAGGCCCCCTTTCTTCGCCTCTTCCTT |
| Internal bar code: | TCTATTCGAGTGATTCTGAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1177 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGGTCATGGGGTCAGTC |
| Suggested primer 2: | CCAGCCCTGATTGCAGTGTA |