Insertion junction: CLIP2.018043_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.018043
Chromosome:chromosome 7
Location:792433
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGCTGATAGAGGTTACATGTGGGACGACAGCCGTGGACTAAGCCAGGGC
Internal bar code:GAGCATTATGTCAATACTGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1256
LEAP-Seq percent confirming:77.2727
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAACTATGTTGGCGGCAA
Suggested primer 2:GGTCATCGCCAATAGAGCCA