Insertion junction: CLIP2.018057_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.018057
Chromosome:chromosome 14
Location:182545
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g608800 NOL1,SDR24 (1 of 1) 1.1.1.294 - Chlorophyll(ide) b reductase / Chlorophyll b reductase; Chlorophyll b reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTTAACTGCAACAGCTGCGCGCCCATTCCGCCTCCACCTCCTTCGCTG
Internal bar code:GACTAGCCGACTTAGGACCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:136
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TACACGGTACACACAAGGCC
Suggested primer 2:ACACACACACACACACACCT