Insertion junction: CLIP2.018072_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.018072
Chromosome:chromosome 7
Location:5385901
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g350400 PIN3 Peptidyl-prolyl cis-trans isomerase, parvulin-type; (1 of 1) 2.7.7.80//2.8.1.11//5.2.1.8 - Molybdopterin-synthase adenylyltransferase // Molybdopterin-synthase sulfurtransferase / Molybdopterin synthase sulfurylase // Peptidylprolyl isomerase / Rotamase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTGGATGCATATAATTGGCCAAGCCCAGAAGGGATGACATGCAGGTCA
Internal bar code:TTTACGATCGTGGTTTTTCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:1690
LEAP-Seq percent confirming:82.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCACGCTTCGTATCTTG
Suggested primer 2:GGGGAATAGAGCTGTCGACG