Insertion junction: CLIP2.018072_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.018072
Chromosome:chromosome 7
Location:5385903
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g350400 PIN3 Peptidyl-prolyl cis-trans isomerase, parvulin-type; (1 of 1) 2.7.7.80//2.8.1.11//5.2.1.8 - Molybdopterin-synthase adenylyltransferase // Molybdopterin-synthase sulfurtransferase / Molybdopterin synthase sulfurylase // Peptidylprolyl isomerase / Rotamase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCCAGGCCACCATCCAGCAGATGAACCCCGAGGAGCTGTACGAGATGA
Internal bar code:TTTACGATCGTGGTTTTTCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:2206
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGAATAGAGCTGTCGACG
Suggested primer 2:GCTGCACGCTTCGTATCTTG