Insertion junction: CLIP2.018109_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.018109
Chromosome:chromosome 7
Location:1800277
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325727 (1 of 3) PTHR10566:SF76 - PROTEIN Y32H12A.7 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTGCCGCCACGGCCGCCGCCAGCACCACCGCCGCCAGCGCCGCCGCCG
Internal bar code:CGCCACGAACTTAGTGTCACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1476
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:GCGATCTATCAGCACGGACA
Suggested primer 2:CACCAACAGCAACTCACTGC