| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.018117 |
| Chromosome: | chromosome 16 |
| Location: | 5958867 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678850 | (1 of 1) 2.3.3.10 - Hydroxymethylglutaryl-CoA synthase / Hydroxymethylglutaryl coenzyme alpha-condensing enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCACCCACTCCTGCGCCCATTCACCAGCTGCACCCCCCTCCAGCGCTG |
| Internal bar code: | CCTTTAATCATATTTACTTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4545 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGACATCGCATCCTCGCC |
| Suggested primer 2: | TCCACAATGCCAACCCCTTT |