Insertion junction: CLIP2.018132_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.018132
Chromosome:chromosome 8
Location:4090528
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g383101 FAP324 (1 of 1) PF00041//PF13418 - Fibronectin type III domain (fn3) // Galactose oxidase, central domain (Kelch_4); Flagellar Associated Protein 324 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCCTCCCTGTATCCGCCCCCCTCCCTGCCCACACTGGATTCGTTGGCT
Internal bar code:CTTTGTATCCGCAGGGAATTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:193
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTCTCTGGGTTCCTGAC
Suggested primer 2:CCCAAGAGCCAAGGAATCGT