Insertion junction: CLIP2.018147_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.018147
Chromosome:chromosome 9
Location:6413408
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g414800 CPY Serine carboxypeptidase; (1 of 1) K16297 - serine carboxypeptidase-like clade II [EC:3.4.16.-] (SCPL-II) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGCAGTAGCAGACAGGGCGGCAGCATTGGCCAGGCGGCGACGGGCGCC
Internal bar code:GCCTCCTCCCCCACGGGTAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:5311
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:124
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:124

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCATAAATGAGCGCAGTG
Suggested primer 2:CGCGTACAAACAAATTGCGC