Insertion junction: CLIP2.018149_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.018149
Chromosome:chromosome 12
Location:1124244
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g491000 (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGGGACACCTCCTCGACCCGTGTCGCAACACCCACCGCAAGTCGTCCA
Internal bar code:TTATCACCTTCGGATAGGATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2594
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGCTTAGTCCAGAGGGAG
Suggested primer 2:GGAGTGAAGGTGTCGGAAGG