Insertion junction: CLIP2.018151_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.018151
Chromosome:chromosome 16
Location:5039070
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g686000 SRS15 (1 of 1) K13096 - splicing factor 4 (SF4); putative RNA-binding protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGAAACTGTTGTGTAGGCAATGAATGGATGGAGGGGAAAACATATAGA
Internal bar code:TGCTGCGAGACGTGCTGAGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1799
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGCCCCAAACTCTGTTGC
Suggested primer 2:ATTCGTTCAACAGGCCAGGT