Insertion junction: CLIP2.018157_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.018157
Chromosome:chromosome 7
Location:1735671
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325718 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTCTGCCATCACGCTCGGGACAGGTGGGGCCCTGGTGACCCAGCAACC
Internal bar code:ACCACTTGGCAGTATTGCACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:3136
LEAP-Seq percent confirming:57.1429
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGTAGGGGAATCAGGGCATC
Suggested primer 2:GCAGCAATGTTGTCAGCACA