Insertion junction: CLIP2.018170_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.018170
Chromosome:chromosome 17
Location:721578
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g700750 GFY3 (1 of 5) PTHR30178 - INNER MEMBRANE PROTEIN YAAH; Putative acetate transporter intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCCGGGGCCTGTGCCAACCGCGCTCCAGACCCCCGCACCAAGAGGCAG
Internal bar code:AGGTCAACTGTGGGGGTATGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2769
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTTCGGGATGACCACTT
Suggested primer 2:TAGGTTCTTGCTGCTGGTGG